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Laboratory Support, Biobanking, and Services (LSB)

Publications

All recent publications: 2024-2020

View Key Publications

 

  1. Mei L, Song Y, Liu X, Li K, Guo X, Liu L, et al. (2024). Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa. Microorganisms. 12(3):13. https://doi.org/10.3390/microorganisms12030562 PMID:38543613
  2. AlKnawy B, Kozlakidis Z, Tarkoma S, Bates D, Honkela A, Crooks G, et al. (2023). Digital public health leadership in the global fight for health security. BMJ Glob Health. 8(2):e011454. https://doi.org/10.1136/bmjgh-2022-011454 PMID:36792230
  3. Bogaert B, Kozlakidis Z, Caboux E, Péron J, Saintingy P (2023). What went right during the COVID crisis: The capabilities of local actors and lasting innovations in oncology care and research. PLOS Glob Public Health. 3(9):e0002366. https://doi.org/10.1371/journal.pgph.0002366 PMID:37747872
  4. Kintossou AK, Blanco-Lopez J, Iguacel I, Pisanu S, Almeida CCB, Steliarova-Foucher E, et al. (2023). Early Life Nutrition Factors and Risk of Acute Leukemia in Children: Systematic Review and Meta-Analysis. Nutrients. 15(17):15. https://doi.org/10.3390/nu15173775 PMID:37686807
  5. Kintossou AK, Villar S, Kozlakidis Z (2023). Immunological considerations for laboratory staff and COVID-19 biosafety. Biosaf Health. 5(2):108–11. https://doi.org/10.1016/j.bsheal.2023.03.001 PMID:37123452
  6. Kozlakidis Z (2023). Promoting Health for Adolescents: An Editorial. Int J Environ Res Public Health. 20(14):6336. https://doi.org/10.3390/ijerph20146336 PMID:37510569
  7. Kozlakidis Z (2023). The incidence of Coronavirus disease 2019 (COVID-19) among vaccinated healthcare workers (HCWs): evidence for protection from hospitalisation from an Indonesian cohort. Lancet Reg Health Southeast Asia. 11:100146. https://doi.org/10.1016/j.lansea.2023.100146 PMID:36643852
  8. Kozlakidis Z, Shi P, Abarbanel G, Klein C, Sfera A (2023). Recent Developments in Protein Lactylation in PTSD and CVD: Novel Strategies and Targets. BioTech (Basel). 12(2):23. https://doi.org/10.3390/biotech12020038 PMID:37218755
  9. Liu NN, Yi CX, Wei LQ, Zhou JA, Jiang T, Hu CC, et al. (2023). The intratumor mycobiome promotes lung cancer progression via myeloid-derived suppressor cells. Cancer Cell. 41(11):1927–1944.e9. https://doi.org/10.1016/j.ccell.2023.08.012 PMID:37738973
  10. Nur Aisyah D, Lokopessy AF, Naman M, Diva H, Manikam L, Adisasmito W, et al. (2023). The Use of Digital Technology for COVID-19 Detection and Response Management in Indonesia: Mixed Methods Study. Interact J Med Res. 12:e41308. https://doi.org/10.2196/41308 PMID:36623206
  11. Satyanarayanan SK, Kozlakidis Z (2023). Editorial: Rising stars in infectious diseases-Surveillance, prevention and treatment: 2022. Front Med (Lausanne). 10:1234922. https://doi.org/10.3389/fmed.2023.1234922 PMID:37469666
  12. Schmid A, Kozlakidis Z, Bledsoe M (2023). Biobanking in the Asia-Pacific Region: The Challenges of International Biospecimen Sharing. Biopreserv Biobank. 21(4):316–7. https://doi.org/10.1089/bio.2023.29124.editorial PMID:37594516
  13. Sfera A, Andronescu L, Britt WG, Himsl K, Klein C, Rahman L, et al. (2023). Receptor-Independent Therapies for Forensic Detainees with Schizophrenia-Dementia Comorbidity. Int J Mol Sci. 24(21):21. https://doi.org/10.3390/ijms242115797 PMID:37958780
  14. Sfera A, Hazan S, Kozlakidis Z, Klein C (2023). Microbiota-derived psychedelics: Lessons from COVID-19. Adv Clin Exp Med. 32(4):395–9. https://doi.org/10.17219/acem/159477 PMID:36753370
  15. Sfera A, Rahman L, Zapata-Martín Del Campo CM, Kozlakidis Z (2023). Long COVID as a Tauopathy: Of “Brain Fog” and “Fusogen Storms”. Int J Mol Sci. 24(16):18. https://doi.org/10.3390/ijms241612648 PMID:37628830
  16. Adisasmito, W., Aisyah, D. N., Medina, P. B., Kozlakidis, Z., Wang, H., Cheong, I. H., . . . Lin, R. T. (2022). Building Back Better: Addressing the Laboratory Readiness for Biodefence and Global Health Security. Task force 6: Global Health Security and COVID-19. Retrieved from
  17. Aisyah, D. N., Kozlakidis, Z., Diva, H., Trimizi, S. N., Sianipar, L. R., Wijayanti, E., . . . Adisasmito, W. (2022). The Spatial-Temporal Distribution of Chronic Lymphatic Filariasis in Indonesia: A 18-Year Registry-Based Analysis. Microbiology Research. 13(4): 681-690. PMID: https://doi.org/10.3390/microbiolres13040049    
  18. Aisyah, D. N., Manikam, L., Kiasatina, T., Naman, M., Adisasmito, W., & Kozlakidis, Z. (2022). The Use of a Health Compliance Monitoring System During the COVID-19 Pandemic in Indonesia: Evaluation Study. JMIR Public Health Surveill. 8(11): e40089. PMID: 36219836 https://doi.org/10.2196/40089           
  19. Aisyah, D. N., Mayadewi, C. A., Budiharsana, M., Solikha, D. A., Ali, P. B., Igusti, G., . . . Manikam, L. (2022). Building on health security capacities in Indonesia: Lessons learned from the COVID-19 pandemic responses and challenges. Zoonoses and Public Health. 69(6): 757-767. PMID: 35618675 https://doi.org/10.1111/zph.12976               
  20. Al Knawy, B., McKillop, M. M., Abduljawad, J., Tarkoma, S., Adil, M., Schaper, L., . . . Rhee, K. (2022). Successfully Implementing Digital Health to Ensure Future Global Health Security During Pandemics: A Consensus Statement. JAMA Netw Open. 5(2): e220214. PMID: 35195701 https://doi.org/10.1001/jamanetworkopen.2022.0214               
  21. Barin, B., Kozlakidis, Z., Ricci, F., Su, L., Tsioutis, C., Welburn, S. C., . . . Lumbers, E. R. (2022). Editorial: Coronavirus Disease (COVID-19): Pathophysiology, Epidemiology, Clinical Management and Public Health Response, Volume II. Front Public Health. 10: 913507. PMID: 35747774 https://doi.org/10.3389/fpubh.2022.913507   
  22. Bogaert, B., Buisson, V., Kozlakidis, Z., & Saintigny, P. (2022). Organisation of cancer care in troubling times: A scoping review of expert guidelines and their implementation during the COVID-19 pandemic. Critical Reviews in Oncology/Hematology. 173: 103656. PMID: 35337970 https://doi.org/10.1016/j.critrevonc.2022.103656        
  23. Casati, S., Ellul, B., Mayrhofer, M. T., Lavitrano, M., Caboux, E., & Kozlakidis, Z. (2022). Paediatric biobanking for health: The ethical, legal, and societal landscape. Front Public Health. 10: 917615. PMID: 36238242 https://doi.org/10.3389/fpubh.2022.917615      
  24. Cheong, I. H., & Kozlakidis, Z. (2022). The Importance of Cancer Biobanks in Low- and Middle-Income Countries. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 147-154.       
  25. Gramatiuk, S., Huppertz, B., Alekseenko, M., Hartl, G., Macheiner, T., Sarkisian, T., . . . Sargsyan, K. (2022). Methods of Implementation and Set-Up of National Biobanking Networks. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 39-46.       
  26. Gramatiuk, S., Sarkisian, T., Kozlakidis, Z., & Sargsyan, K. (2022). Governance and Stakeholder Analysis. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 73-79.       
  27. Gupta, R. K., & Kozlakidis, Z. O. (2022). Emerging Markets and Technologies: A Special Issue and a New Section for Biopreservation and Biobanking. Biopreservation and Biobanking. 20(5): 415-416. PMID: 36301144 https://doi.org/10.1089/bio.2022.29112.zjk       
  28. He, Y. Q., Wang, T. M., Ji, M., Mai, Z. M., Tang, M., Wang, R., . . . Jia, W. H. (2022). A polygenic risk score for nasopharyngeal carcinoma shows potential for risk stratification and personalized screening. Nat Commun. 13(1): 1966. PMID: 35414057 https://doi.org/10.1038/s41467-022-29570-4              
  29. Karanovic, S., Ardin, M., Tang, Z., Tomic, K., Villar, S., Renard, C., . . . Zavadil, J. (2022). Molecular profiles and urinary biomarkers of upper tract urothelial carcinomas associated with aristolochic acid exposure. International Journal of Cancer. 150(2): 374-386. PMID: 34569060 https://doi.org/10.1002/ijc.33827      
  30. Kozlakidis, Z. (2022). Evidence for Recombination as an Evolutionary Mechanism in Coronaviruses: Is SARS-CoV-2 an Exception? Front Public Health. 10: 859900. PMID: 35372203 https://doi.org/10.3389/fpubh.2022.859900
  31. Kozlakidis, Z., & Gupta, R. K. (2022). Call for Papers: Emerging Markets and Technologies. Biopreservation and Biobanking. 20(1): 1. PMID: 35175834 https://doi.org/10.1089/bio.2021.29101.zjk       
  32. Kozlakidis, Z., & Struelens, M. J. (2022). Editorial: Insights in coronavirus disease (COVID-19) – surveillance, prevention and treatment. Front Public Health. 10: 998998. PMID: 36249198 https://doi.org/10.3389/fpubh.2022.998998
  33. Kozlakidis, Z., & Vandenberg, O. (2022). Maintaining a Focus on Biobanking Science and Innovation. Biopreservation and Biobanking. 20(3): 209-210. PMID: 35703952 https://doi.org/10.1089/bio.2022.29108.zjk       
  34. Liu, N. N., Jiao, N., Tan, J. C., Wang, Z., Wu, D., Wang, A. J., . . . Wang, H. (2022). Multi-kingdom microbiota analyses identify bacterial-fungal interactions and biomarkers of colorectal cancer across cohorts. Nat Microbiol. 7(2): 238-250. PMID: 35087227 https://doi.org/10.1038/s41564-021-01030-7    
  35. Matera-Witkiewicz, A., Zagorska, K., Kozlakidis, Z., & Glenska-Olender, J. (2022). Letter to the Editor: Creation of National Guides in the Frame of International Standards and Best Practices in Biobanking: “Quality Standards for Polish Biobanks Handbook”. Biopreservation and Biobanking. 20(6): 575-576. PMID: 35363056 https://doi.org/10.1089/bio.2021.0111
  36. Mitchell, C., Gramatiuk, S., Sarkisian, T., Kozlakidis, Z., & Sargsyan, K. (2022). Biobanking IT Systems, Database Structure and Web Applications. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 81-89.  
  37. Ngwa, W., Addai, B. W., Adewole, I., Ainsworth, V., Alaro, J., Alatise, O. I., . . . Kerr, D. (2022). Cancer in sub-Saharan Africa: a Lancet Oncology Commission. Lancet Oncology. 23(6): e251-e312. PMID: 35550267 https://doi.org/10.1016/S1470-2045(21)00720-8               
  38. Osorio, C., Sfera, A., Anton, J. J., Thomas, K. G., Andronescu, C. V., Li, E., . . . Kozlakidis, Z. (2022). Virus-Induced Membrane Fusion in Neurodegenerative Disorders. Front Cell Infect Microbiol. 12: 845580. PMID: 35531328 https://doi.org/10.3389/fcimb.2022.845580       
  39. Sargsyan, K., Gramatiuk, S., Alekseenko, M., Macheiner, T., Hartl, G., Sarkisian, T., . . . Huppertz, B. (2022). Collection and Management of Samples. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 57-63.    
  40. Sfera, A., Hazan, S., Anton, J. J., Sfera, D. O., Andronescu, C. V., Sasannia, S., . . . Kozlakidis, Z. (2022). Psychotropic drugs interaction with the lipid nanoparticle of COVID-19 mRNA therapeutics. Frontiers in Pharmacology. 13: 995481. PMID: 36160443 https://doi.org/10.3389/fphar.2022.995481       
  41. Sfera, A., Thomas, K. G., Sasannia, S., Anton, J. J., Andronescu, C. V., Garcia, M., . . . Kozlakidis, Z. (2022). Neuronal and Non-Neuronal GABA in COVID-19: Relevance for Psychiatry. Reports. 5(2): 20. PMID: https://doi.org/10.3390/reports5020022      
  42. Shirakashi, R., Kozlakidis, Z., Yadav, B. K., Ng, W., Fachiroh, J., Vu, H., . . . Furuta, K. O. (2022). Decarbonization in Biobanking: A Potential New Scientific Area. Biopreservation and Biobanking. 20(5): 446-450. PMID: 36301139 https://doi.org/10.1089/bio.2022.0146               
  43. Simeon-Dubach, D., & Kozlakidis, Z. (2022). Sustainability of Biobanks and Biobanking in LMICs. In: K. Sargsyan, B. Huppertz, & S. Gramatiuk, editor.^editors. Biobanks in Low- and Middle-Income Countries: Relevance, Setup and Management. ed. Cham: Springer International Publishing; pp. 193-200.   
  44. Stejskal, L., Kalemera, M. D., Lewis, C. B., Palor, M., Walker,
  45. Aisyah, D. N., Mayadewi, C. A., Igusti, G., Manikam, L., Adisasmito, W., & Kozlakidis, Z. (2021). Laboratory Readiness and Response for SARS-Cov-2 in Indonesia. Front Public Health. 9: 705031. PMID: 34350153  doi: 10.3389/fpubh.2021.705031
  46. Al Knawy, B., & Kozlakidis, Z. (2021). ‘Quaranta giorni’ leadership test: time to transform healthcare. Bmj Leader. 5(1): 48-50. doi: 10.1136/leader-2020-000276
  47. Cree, I. A., Indave Ruiz, B. I., Zavadil, J., McKay, J., Olivier, M., Kozlakidis, Z., . . . participants, I. R. (2021). The International Collaboration for Cancer Classification and Research. Int J Cancer. 148(3): 560-571. PMID: 32818326  doi: 10.1002/ijc.33260
  48. Doolan, D. L., Kozlakidis, Z., Zhang, Z., Paessler, S., Su, L., Yokota, Y. T., . . . Zhang, S. Y. (2021). Editorial: Coronavirus Disease (COVID-19): Pathophysiology, Epidemiology, Clinical Management and Public Health Response. Front Public Health. 9: 807159. PMID: 34917586  doi: 10.3389/fpubh.2021.807159
  49. Garcia, D. L., & Kozlakidis, Z. (2021). Letter to the editor: ISBER Two Decades and Beyond: Honoring Our Past, Celebrating the Present, and Envisioning Our Future. Biopreserv Biobank. 19(4): 353-354. PMID: 34129381  doi: 10.1089/bio.2021.29088.dlg
  50. Henderson, M. K., Afifi, N., & Kozlakidis, Z. (2021). Zooming Along Through the Pandemic: Our Experiences with Virtual Biobanking Conferences and Workshops. Biopreserv Biobank. 19(4): 247-249. PMID: 34348044  doi: 10.1089/bio.2021.29091.mkh
  51. Roux, J., Zeghidi, M., Villar, S., & Kozlakidis, Z. (2021). Biosafety and biobanking: Current understanding and knowledge gaps. Biosaf Health. 3(5): 244-248. PMID: 34179747  doi: 10.1016/j.bsheal.2021.06.003
  52. Sfera, A., Osorio, C., Rahman, L., Zapata-Martin Del Campo, C. M., Maldonado, J. C., Jafri, N., . . . Kozlakidis, Z. (2021). PTSD as an Endothelial Disease: Insights From COVID-19. Front Cell Neurosci. 15: 770387. PMID: 34776871  doi: 10.3389/fncel.2021.770387
  53. Sfera, A., Osorio, C., Zapata Martin Del Campo, C. M., Pereida, S., Maurer, S., Maldonado, J. C., & Kozlakidis, Z. (2021). Endothelial Senescence and Chronic Fatigue Syndrome, a COVID-19 Based Hypothesis. Front Cell Neurosci. 15: 673217. PMID: 34248502  doi: 10.3389/fncel.2021.673217
  54. Vandenberg, O., Martiny, D., Rochas, O., van Belkum, A., & Kozlakidis, Z. (2021). Considerations for diagnostic COVID-19 tests. Nat Rev Microbiol. 19(3): 171-183. PMID: 33057203  doi: 10.1038/s41579-020-00461-z
  55. Vodosin, P., Jorgensen, A. K., Mendy, M., Kozlakidis, Z., Caboux, E., & Zawati, M. H. (2021). A Review of Regulatory Frameworks Governing Biobanking in the Low and Middle Income Member Countries of BCNet. Biopreserv Biobank. 19(5): 444-452. PMID: 33945303  doi: 10.1089/bio.2020.0101
  56. Wei, L. Q., Cheong, I. H., Yang, G. H., Li, X. G., Kozlakidis, Z., Ding, L., . . . Wang, H. (2021). The Application of High-Throughput Technologies for the Study of Microbiome and Cancer. Front Genet. 12: 699793. PMID: 34394190  doi: 10.3389/fgene.2021.699793
  57. Afifi, N. M., Anisimov, S. V., Aguilar-Quesada, R., Kinkorova, J., Marrs, S., Nassimbwa, S., . . . Parry-Jones, A. (2020). Biobanking Spotlight on Europe, Middle East, and Africa: Presenting the Collective Experience of the ISBER-EMEA Regional Ambassadors. Biopreserv Biobank. 18(5): 471-478. PMID: 32780585 doi: 10.1089/bio.2020.0013
  58. Aisyah, D. N., Ahmad, R. A., Artama, W. T., Adisasmito, W., Diva, H., Hayward, A. C., & Kozlakidis, Z. (2020). Knowledge, Attitudes, and Behaviors on Utilizing Mobile Health Technology for TB in Indonesia: A Qualitative Pilot Study. Front Public Health. 8: 531514. PMID: 33123508 doi: 10.3389/fpubh.2020.531514
  59. Aisyah, D. N., Mayadewi, C. A., Diva, H., Kozlakidis, Z., Siswanto, & Adisasmito, W. (2020). A spatial-temporal description of the SARS-CoV-2 infections in Indonesia during the first six months of outbreak. PLoS ONE. 15(12): e0243703. PMID: 33351801 doi: 10.1371/journal.pone.0243703
  60. Allocca, C. M., Bledsoe, M. J., Albert, M., Anisimov, S. V., Bravo, E., Castelhano, M. G., . . . Snapes, E. (2020). Biobanking in the COVID-19 Era and Beyond: Part 1. How Early Experiences Can Translate into Actionable Wisdom. Biopreserv Biobank. 18(6): 533-546. PMID: 33164554 doi: 10.1089/bio.2020.0082
  61. Allocca, C. M., Snapes, E., Albert, M., Bledsoe, M. J., Castelhano, M. G., De Wilde, M., . . . Schacter, B. (2020). Biobanking in the COVID-19 Era and Beyond: Part 2. A Set of Tool Implementation Case Studies. Biopreserv Biobank. 18(6): 547-560. PMID: 33226280 doi: 10.1089/bio.2020.0083
  62. Di Lorenzo, G., Buonerba, L., Ingenito, C., Crocetto, F., Buonerba, C., Libroia, A., . . . De Placido, S. (2020). Clinical Characteristics of Metastatic Prostate Cancer Patients Infected with COVID-19 in South Italy. Oncology. 98(10): 743-747. PMID: 32570240 doi: 10.1159/000509434
  63. Di Lorenzo, G., Di Trolio, R., Kozlakidis, Z., Busto, G., Ingenito, C., Buonerba, L., . . . Leo, E. (2020). COVID 19 therapies and anti-cancer drugs: A systematic review of recent literature. Crit Rev Oncol Hematol. 152: 102991. PMID: 32544802 doi: 10.1016/j.critrevonc.2020.102991
  64. Eichler, S. E., Hopperton, A. P., Alava, J. J., Pereira, A., Jr., Ahmed, R., Kozlakidis, Z., . . . Rodriguez-Palacios, A. (2020). A Citizen Science Facemask Experiment and Educational Modules to Improve Coronavirus Safety in Communities and Schools. Front Med (Lausanne). 7: 486. PMID: 33015086 doi: 10.3389/fmed.2020.00486
  65. Eklund, N., Andrianarisoa, N. H., van Enckevort, E., Anton, G., Debucquoy, A., Muller, H., . . . Silander, K. (2020). Extending the Minimum Information About BIobank Data Sharing Terminology to Describe Samples, Sample Donors, and Events. Biopreserv Biobank. 18(3): 155-164. PMID: 32302498 doi: 10.1089/bio.2019.0129
  66. Henderson, M. K., & Kozlakidis, Z. (2020). Coronavirus and Biobanking: The Collective Global Experiences of the First Wave and Bracing During the Second. Biopreserv Biobank. 18(6): 481-482. PMID: 33147072 doi: 10.1089/bio.2020.29077.mjk
  67. Henderson, M. K., Kozlakidis, Z., Fachiroh, J., Wiafe Addai, B., Xu, X., Ezzat, S., . . . Yadav, B. K. (2020). The Responses of Biobanks to COVID-19. Biopreserv Biobank. 18(6): 483-491. PMID: 32871085 doi: 10.1089/bio.2020.29074.mkh
  68. Jazieh, A. R., & Kozlakidis, Z. (2020). Healthcare Transformation in the Post-Coronavirus Pandemic Era. Front Med (Lausanne). 7: 429. PMID: 32850915 doi: 10.3389/fmed.2020.00429
  69. Kozlakidis, Z., Abduljawad, J., Al Khathaami, A. M., Schaper, L., & Stelling, J. (2020). Global health and data-driven policies for emergency responses to infectious disease outbreaks. Lancet Glob Health. 8(11): e1361-e1363. PMID: 32791118 doi: 10.1016/S2214-109X(20)30361-2
  70. Kozlakidis, Z., Vandenberg, O., & Stelling, J. (2020). Editorial: Clinical Microbiology in Low Resource Settings. Front Med (Lausanne). 7: 258. PMID: 32587861 doi: 10.3389/fmed.2020.00258
  71. O’Donoghue, S., Byrne, J. A., Green, C., Hill, K., Kozlakidis, Z., De Wilde, A., . . . Wiles, K. R. (2020). The Experts Speak on Biobank Education. Biopreserv Biobank. 18(1): 14-17. PMID: 32069096 doi: 10.1089/bio.2020.29064.sod
  72. Shimakawa, Y., Ndow, G., Njie, R., Njai, H. F., Takahashi, K., Akbar, S. M. F., . . . Lemoine, M. (2020). Hepatitis B Core-related Antigen: An Alternative to Hepatitis B Virus DNA to Assess Treatment Eligibility in Africa. Clin Infect Dis. 70(7): 1442-1452. PMID: 31102406 doi: 10.1093/cid/ciz412
  73. Vandenberg, O., Durand, G., Hallin, M., Diefenbach, A., Gant, V., Murray, P., . . . van Belkum, A. (2020). Consolidation of Clinical Microbiology Laboratories and Introduction of Transformative Technologies. Clin Microbiol Rev. 33(2): e00057-00019. PMID: 32102900 doi: 10.1128/cmr.00057-19

 

 
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